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J Cell Biol. 2017 Nov 6;216(11):3591-3608. doi: 10.1083/jcb.201612194. Epub 2017 Oct 3.

The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.

Author information

1
Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide-Consejo Superior de Investigaciones Científicas, Junta de Andalucia, Seville, Spain.
2
Department of Physiological Chemistry, Biomedical Center Munich, Ludwig-Maximilians-Universität München, Planegg-Martiensried, Germany.
3
International Max Planck Research School for Molecular and Cellular Life Sciences, Planegg-Martinsried, Germany.
4
Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide-Consejo Superior de Investigaciones Científicas, Junta de Andalucia, Seville, Spain rroddag@upo.es.

Abstract

Kinetochores (KTs) are large multiprotein complexes that constitute the interface between centromeric chromatin and the mitotic spindle during chromosome segregation. In spite of their essential role, little is known about how centromeres and KTs are assembled and how their precise stoichiometry is regulated. In this study, we show that the nuclear pore basket component Alm1 is required to maintain both the proteasome and its anchor, Cut8, at the nuclear envelope, which in turn regulates proteostasis of certain inner KT components. Consistently, alm1-deleted cells show increased levels of KT proteins, including CENP-CCnp3, spindle assembly checkpoint activation, and chromosome segregation defects. Our data demonstrate a novel function of the nucleoporin Alm1 in proteasome localization required for KT homeostasis.

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PMID:
28974540
PMCID:
PMC5674884
DOI:
10.1083/jcb.201612194
[Indexed for MEDLINE]
Free PMC Article

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