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Expert Rev Proteomics. 2017 Nov;14(11):1037-1053. doi: 10.1080/14789450.2017.1388167. Epub 2017 Oct 13.

Protein purification and analysis: next generation Western blotting techniques.

Author information

1
a Department of Physiology , University of Saskatchewan College of Medicine , Saskatoon , SK , Canada.
2
b Department of Neurobiology, Physiology, and Behavior , University of California , Davis , CA , USA.
3
c Department of Physiology and Membrane Biology , University of California , Davis , CA , USA.

Abstract

Western blotting is one of the most commonly used techniques in molecular biology and proteomics. Since western blotting is a multistep protocol, variations and errors can occur at any step reducing the reliability and reproducibility of this technique. Recent reports suggest that a few key steps, such as the sample preparation method, the amount and source of primary antibody used, as well as the normalization method utilized, are critical for reproducible western blot results. Areas covered: In this review, improvements in different areas of western blotting, including protein transfer and antibody validation, are summarized. The review discusses the most advanced western blotting techniques available and highlights the relationship between next generation western blotting techniques and its clinical relevance. Expert commentary: Over the last decade significant improvements have been made in creating more sensitive, automated, and advanced techniques by optimizing various aspects of the western blot protocol. New methods such as single cell-resolution western blot, capillary electrophoresis, DigiWest, automated microfluid western blotting and microchip electrophoresis have all been developed to reduce potential problems associated with the western blotting technique. Innovative developments in instrumentation and increased sensitivity for western blots offer novel possibilities for increasing the clinical implications of western blot.

KEYWORDS:

Immunoblot; Western blotting; Western blotting techniques; next generation Western blotting; protein purification; protein sample preparation

PMID:
28974114
PMCID:
PMC6810642
DOI:
10.1080/14789450.2017.1388167
[Indexed for MEDLINE]
Free PMC Article

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