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Proc Natl Acad Sci U S A. 2017 Oct 10;114(41):10984-10989. doi: 10.1073/pnas.1704835114. Epub 2017 Sep 25.

Drk-mediated signaling to Rho kinase is required for anesthesia-resistant memory in Drosophila.

Author information

1
Division of Neuroscience, Biomedical Sciences Research Centre "Alexander Fleming," Vari 16672, Greece.
2
Division of Neuroscience, Biomedical Sciences Research Centre "Alexander Fleming," Vari 16672, Greece skoulakis@fleming.gr.

Abstract

Anesthesia-resistant memory (ARM) was described decades ago, but the mechanisms that underlie this protein synthesis-independent form of consolidated memory in Drosophila remain poorly understood. Whether the several signaling molecules, receptors, and synaptic proteins currently implicated in ARM operate in one or more pathways and how they function in the process remain unclear. We present evidence that Drk, the Drosophila ortholog of the adaptor protein Grb2, is essential for ARM within adult mushroom body neurons. Significantly, Drk signals engage the Rho kinase Drok, implicating dynamic cytoskeletal changes in ARM, and this is supported by reduced F-actin in the mutants and after pharmacological inhibition of Drok. Interestingly, Drk-Drok signaling appears independent of the function of Radish (Rsh), a protein long implicated in ARM, suggesting that the process involves at least two distinct molecular pathways. Based on these results, we propose that signaling pathways involved in structural plasticity likely underlie this form of translation-independent memory.

KEYWORDS:

ARM; Drk; Drok; Drosophila; memory

PMID:
28973902
PMCID:
PMC5642687
DOI:
10.1073/pnas.1704835114
[Indexed for MEDLINE]
Free PMC Article

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