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J Med Microbiol. 2017 Oct;66(10):1436-1442. doi: 10.1099/jmm.0.000603. Epub 2017 Oct 4.

Prevalence and genetic diversity of Trichomonas vaginalis in the general population of Granada and co-infections with Gardnerella vaginalis and Candida species.

Author information

1
1​Biobank of the Andalusian Public Health System (BBSSPA), Granada, Spain.
2
2​Microbiology Laboratory, Virgen de las Nieves University Hospital -ibs Granada, Granada, Spain.
3
3​Department of Obstetrics and Gynaecology, Virgen de las Nieves University Hospital -ibs Granada, Granada, Spain.
4
4​Department of Microbiology, University of Granada-ibs Granada, Granada, Spain.
5
5​Department of Microbiology and Infection, Statens Serum Institut, Copenhagen, Denmark.

Abstract

PURPOSE:

Purulent or exudative genitourinary infections are a frequent cause of consultation in primary and specialized healthcare. The objectives of this study were: to determine the prevalence of Trichomonas vaginalis and co-infections with Candida spp. and Gardnerella vaginalis in vaginal secretion; and to use multilocus sequence typing (MLST) to analyse the genetic diversity of T. vaginalis strains.

METHODOLOGY:

The samples were submitted for analysis (n=5230) to a third-level hospital in Granada (Southern Spain) between 2011 and 2014; eight T. vaginalis strains isolated during 2015 were randomly selected for MLST analysis. Culture and nucleic acid hybridization techniques were used to detect microorganisms in the samples.

RESULTS:

The prevalence of T. vaginalis was 2.4 % between 2011 and 2014, being higher during the first few months of both 2011 and 2012. Among samples positive for T. vaginalis, co-infection with G. vaginalis was detected in 29 samples and co-infection with Candida spp. in 6, while co-infection with all three pathogens was observed in 3 samples. The only statistically significant between-year difference in co-infection rates was observed for T. vaginalis with G. vaginalis due to an elevated rate in 2011. MLST analysis results demonstrated a high genetic variability among strains circulating in our setting.

CONCLUSION:

These findings emphasize the need for the routine application of diagnostic procedures to avoid the spread of this sexually transmitted infection.

KEYWORDS:

Candida; Gardnerella vaginalis; MLST; Trichomonas vaginalis; Vaginitis

PMID:
28972465
DOI:
10.1099/jmm.0.000603
[Indexed for MEDLINE]

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