Send to

Choose Destination
Neuroscience. 1988 Jan;24(1):227-36.

Co-localization of neurotensin- and cholecystokinin-like immunoreactivities in catecholamine neurons in the rat dorsomedial medulla.

Author information

2nd Department of Anatomy, Osaka University Medical School, Japan.


Co-localization of neurotensin and cholecystokinin in tyrosine hydroxylase-containing neurons in the nucleus tractus solitarius of the rat was demonstrated by immunocytochemistry with fluorescent double-staining combined with the peroxidase-antiperoxidase method. Co-localization of neurotensin/tyrosine hydroxylase or cholecystokinin/tyrosine hydroxylase was consistently found in small neurons in the region dorsomedial to the tractus solitarius at the level of the area postrema with high percentages of co-existence: 91.0% tyrosine hydroxylase-immunoreactive neurons contained neurotensin and 91.1% cholecystokinin, suggesting that they represent the same neurons. Accordingly, co-localization of neurotensin and cholecystokinin was assessed on tyrosine hydroxylase-containing neurons bisected into two adjacent sections, and then identified in a certain number of the catecholamine neurons in this region. Furthermore these catecholamine neurons exhibited immunoreactivity for an adrenaline-synthesizing enzyme, phenylethanolamine N-methyltransferase. It was concluded that catecholamine, in particular adrenaline, neurons, characterized by co-localization of neurotensin and cholecystokinin, established a distinct subpopulation in the catecholaminergic system in the dorsomedial medulla of the rat.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center