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Int J Occup Environ Med. 2017 Oct;8(4):224-231. doi: 10.15171/ijoem.2017.1089.

Primary DNA Damage in Dry Cleaners with Perchlorethylene Exposure.

Author information

1
Department of Occupational Health, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
2
International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
3
Department of Medical Biochemistry, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
4
Department of Occupational Health, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. mjzs63@gmail.com.
5
Department of Occupational Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Abstract

BACKGROUND:

Perchloroethylene is a halogenated solvent widely used in dry cleaning. International agency of research on cancer classified this chemical as a probable human carcinogen.

OBJECTIVE:

To evaluate the extent of primary DNA damage in dry cleaner workers who were exposed to perchloroethylene as compared to non-exposed subjects. The effect of exposure modifying factors such as use of personal protective equipment, perceived risk, and reported safe behaviors on observed DNA damage were also studied.

METHODS:

59 exposed and non-exposed workers were selected from Yazd, Iran. All the 33 exposed workers had work history at least 3 months in the dry cleaning shops. Peripheral blood sampling was performed. Microscope examination was performed under fluorescent microscope (400×). Open comet software was used for image analysis. All biological analysis was performed in one laboratory.

RESULTS:

Primary DNA damage to leukocytes in dry cleaners was relatively high. The median tail length, %DNA in tail, and tail moment in exposed group were significantly higher than those in non-exposed group. There was no significant difference between smokers and nonsmokers in terms of tail length, tail moment, and %DNA in tail. There was no significant correlation between duration of employment in dry cleaning and observed DNA damage in terms of tail length, tail moment and %DNA in tail. Stratified analysis based on exposed and nonexposed category showed no significant relationship between age and observed DNA damage.

CONCLUSION:

Occupationally exposure to perchloroethylene can cause early DNA damage in dry cleaners.

KEYWORDS:

Clothing; Comet assay; DNA damage; Occupational exposure

PMID:
28970597
PMCID:
PMC6679606
DOI:
10.15171/ijoem.2017.1089
[Indexed for MEDLINE]
Free PMC Article

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