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Biosystems. 2017 Dec;162:119-127. doi: 10.1016/j.biosystems.2017.09.014. Epub 2017 Sep 29.

Cyclic decomposition explains a photosynthetic down regulation for Chlamydomonas reinhardtii.

Author information

1
Faculty of Biology, Medicine and Health, University of Manchester, Oxford Road, Manchester, M13 9PT, UK; School of Earth and Environmental Sciences, University of Manchester, Oxford Road, Manchester, M13 9PT, UK; Current address: Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Gogerrddan, Aberystwyth, SY23 3EE, UK.
2
Laboratório Nacional de Computação Científica, Petrópolis, RJ, 25651-075, Brazil.
3
School of Earth and Environmental Sciences, University of Manchester, Oxford Road, Manchester, M13 9PT, UK.
4
Faculty of Biology, Medicine and Health, University of Manchester, Oxford Road, Manchester, M13 9PT, UK. Electronic address: jean-marc.schwartz@manchester.ac.uk.

Abstract

The regulation of metabolic networks has been shown to be distributed and shared through the action of metabolic cycles. Biochemical cycles play important roles in maintaining flux and substrate availability for multiple pathways to supply cellular energy and contribute to dynamic stability. By understanding the cyclic and acyclic flows of matter through a network, we are closer to understanding how complex dynamic systems distribute flux along interconnected pathways. In this work, we have applied a cycle decomposition algorithm to a genome-scale metabolic model of Chlamydomonas reinhardtii to analyse how acetate supply affects the distribution of fluxes that sustain cellular activity. We examined the role of metabolic cycles which explain the down regulation of photosynthesis that is observed when cells are grown in the presence of acetate. Our results suggest that acetate modulates changes in global metabolism, with the pentose phosphate pathway, the Calvin-Benson cycle and mitochondrial respiration activity being affected whilst reducing photosynthesis. These results show how the decomposition of metabolic flux into cyclic and acyclic components helps to understand the impact of metabolic cycling on organismal behaviour at the genome scale.

KEYWORDS:

Acetate metabolism; Cyclic electron flow; Flux balance analysis; Glycolysis; Green algae; Metabolic model; Mixotrophic growth; Photosynthesis

PMID:
28970020
PMCID:
PMC5720477
DOI:
10.1016/j.biosystems.2017.09.014
[Indexed for MEDLINE]
Free PMC Article

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