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J Pediatr. 2018 Jan;192:171-177.e1. doi: 10.1016/j.jpeds.2017.08.062. Epub 2017 Sep 29.

Crying Time and RORγ/FOXP3 Expression in Lactobacillus reuteri DSM17938-Treated Infants with Colic: A Randomized Trial.

Author information

1
Department of Pediatrics, Universitary Hospital Città della Salute e della Scienza di Torino, Turin, Italy. Electronic address: francesco.savino@unito.it.
2
Department of Pediatrics, Universitary Hospital Città della Salute e della Scienza di Torino, Turin, Italy.
3
Department of Public Health and Pediatric Sciences, University of Turin, School of Medicine, Turin, Italy.

Abstract

OBJECTIVES:

To evaluate crying time, retinoid-related orphan receptor-γ (RORγ) and forkhead box P3 (FOXP3) messenger RNA levels (transcription factors that can modulate T cell responses to gut microbes), and to investigate gut microbiota and fecal calprotectin in infants treated with Lactobacillus reuteri for infantile colic.

STUDY DESIGN:

A double-blind, placebo-controlled randomized trial was conducted in primary care in Torino from August 1, 2015 to September 30, 2016. Patients suffering from infantile colic were randomly assigned to receive daily oral L reuteri (1 × 108 colony forming unit) or placebo for 1 month. Daily crying times were recorded in a structured diary. FOXP3 and RORγ messenger RNA in the peripheral blood was assessed with real-time TaqMan reverse transcription polymerase chain reaction. Gut microbiota and fecal calprotectin were evaluated.

RESULTS:

After infants with colic were supplemented with L reuteri DSM 17938 for 30 days, crying times were significantly shorter among infants with colic in the probiotic group compared with infants in the placebo group (74.67 ± 25.04 [IQR = 79] minutes /day vs 147.85 [IQR = 135] minutes /day [P = .001]). The FOXP3 concentration increased significantly (P = .009), resulting in decreased RORγ/FOXP3 ratios: 0.61 (IQR = 0.60) at day 0 and 0.48 (IQR = 0.28) at day 30 (P = .028). Furthermore, the probiotic increased the percentage of Lactobacillus (P = .049) and decreased fecal calprotectin (P = .0001).

CONCLUSIONS:

Infants with colic treated with L reuteri for 30 days had a significantly decreased crying time and an increased FOXP3 concentration, resulting in a decreased RORγ/FOXP3 ratio. The treatment reduced fecal calprotectin.

TRIAL REGISTRATION:

ClinicalTrials.gov: NCT00893711.

KEYWORDS:

FOXP3; RORγ; Th17/Treg balance; fecal calprotectin; gut microbiota; infantile colic; probiotic

PMID:
28969887
DOI:
10.1016/j.jpeds.2017.08.062
[Indexed for MEDLINE]
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