Format

Send to

Choose Destination
Chem Senses. 2017 Oct 31;42(9):759-767. doi: 10.1093/chemse/bjx055.

Type III Cells in Anterior Taste Fields Are More Immunohistochemically Diverse Than Those of Posterior Taste Fields in Mice.

Wilson CE1,2,3, Finger TE1,3,4, Kinnamon SC1,2,3.

Author information

1
Rocky Mountain Taste and Smell Center, University of Colorado School of Medicine, Aurora, CO 80045, USA.
2
Department of Otolaryngology, University of Colorado School of Medicine, Aurora, CO 80045, USA.
3
Neuroscience Graduate Program, University of Colorado School of Medicine, Aurora, CO 80045, USA.
4
Department of Cell and Developmental Biology, University of Colorado School of Medicine, Aurora, CO 80045, USA.

Abstract

Activation of Type III cells in mammalian taste buds is implicated in the transduction of acids (sour) and salty stimuli. Several lines of evidence suggest that function of Type III cells in the anterior taste fields may differ from that of Type III cells in posterior taste fields. Underlying anatomy to support this observation is, however, scant. Most existing immunohistochemical data characterizing this cell type focus on circumvallate taste buds in the posterior tongue. Equivalent data from anterior taste fields-fungiform papillae and soft palate-are lacking. Here, we compare Type III cells in four taste fields: fungiform, soft palate, circumvallate, and foliate in terms of reactivity to four canonical markers of Type III cells: polycystic kidney disease 2-like 1 (PKD2L1), synaptosomal associated protein 25 (SNAP25), serotonin (5-HT), and glutamate decarboxylase 67 (GAD67). Our findings indicate that while PKD2L1, 5-HT, and SNAP25 are highly coincident in posterior taste fields, they diverge in anterior taste fields. In particular, a subset of taste cells expresses PKD2L1 without the synaptic markers, and a subset of SNAP25 cells lacks expression of PKD2L1. In posterior taste fields, GAD67-positive cells are a subset of PKD2L1 expressing taste cells, but anterior taste fields also contain a significant population of GAD67-only expressing cells. These differences in expression patterns may underlie the observed functional differences between anterior and posterior taste fields.

KEYWORDS:

GABA; PKD2L1; fungiform; salty; serotonin; sour

PMID:
28968659
PMCID:
PMC5863558
DOI:
10.1093/chemse/bjx055
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center