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Zebrafish. 2017 Dec;14(6):547-551. doi: 10.1089/zeb.2017.1508. Epub 2017 Oct 2.

Report of Workshop on Euthanasia for Zebrafish-A Matter of Welfare and Science.

Author information

1
1 Safety and Environment, Karlsruhe Institute of Technology (KIT) , Karlsruhe, Germany .
2
2 Division of Comparative Medicine, University of Toronto , Toronto, Canada .
3
3 Department of Genes-Circuits-Behavior, Max Planck Institute of Neurobiology , Martinsried, Germany .
4
4 European Zebrafish Resource Center, Karlsruhe Institute of Technology (KIT) , Karlsruhe, Germany .
5
5 European Society for Fish Models in Biology and Medicine, Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT) , Karlsruhe, Germany .
6
6 Institute of Integrative Biology, University of Liverpool , Liverpool, United Kingdom .
7
7 Faculty of Medicine, Institute for Cardiovascular Organogenesis and Regeneration, University of Münster , Münster, Germany .
8
8 i3S-Instituto de Investigação e Inovação em Saúde and IBMC-Instituto de Biologia Molecular e Celular , Universidade do Porto, Porto, Portugal .
9
9 CITAB-Centro de Investigação e Tecnologias Agroambientais e Biológicas, Universidade de Trás-os-Montes e Alto Douro, Vila Real, Portugal .
10
10 Zebrafish International Resource Center, Institute of Neuroscience, University of Oregon , Eugene, Oregon.
11
11 Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT) , Karlsruhe, Germany .

Abstract

The increasing importance of zebrafish as a biomedical model organism is reflected by the steadily growing number of publications and laboratories working with this species. Regulatory recommendations for euthanasia as issued in Directive 2010/63/EU are, however, based on experience with fish species used for food production and do not take the small size and specific physiology of zebrafish into account. Consequently, the currently recommended methods of euthanasia in the Directive 2010/63/EU are either not applicable or may interfere with research goals. An international workshop was held in Karlsruhe, Germany, March 9, 2017, to discuss and propose alternative methods for euthanasia of zebrafish. The aim was to identify methods that adequately address the physiology of zebrafish and its use as a biomedical research model, follow the principles of the 3Rs (Replacement, Reduction, and Refinement) in animal experimentation and consider animal welfare during anesthesia and euthanasia. The results of the workshop are summarized here in the form of a white paper.

KEYWORDS:

Directive 2010/63/EU; anesthetics; euthanasia; hypothermic shock; rapid cooling; zebrafish

PMID:
28968196
PMCID:
PMC5706616
DOI:
10.1089/zeb.2017.1508
[Indexed for MEDLINE]
Free PMC Article

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