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Nat Methods. 2017 Nov;14(11):1079-1082. doi: 10.1038/nmeth.4459. Epub 2017 Oct 2.

NeuBtracker-imaging neurobehavioral dynamics in freely behaving fish.

Author information

1
Institute of Biological and Medical Imaging, Helmholtz Zentrum München, Munich, Germany.
2
Institute of Developmental Genetics, Helmholtz Zentrum München, Munich, Germany.
3
Department of Nuclear Medicine, Technical University of Munich, Munich, Germany.
4
Computer Aided Medical Procedures, Technical University of Munich, Munich, Germany.
5
Institute of Neuroscience, Technical University of Munich, Munich, Germany.
6
Champalimaud Centre for the Unknown, Lisbon, Portugal.
7
Chair for Biological Imaging, Technical University of Munich, Munich, Germany.

Abstract

A long-standing objective in neuroscience has been to image distributed neuronal activity in freely behaving animals. Here we introduce NeuBtracker, a tracking microscope for simultaneous imaging of neuronal activity and behavior of freely swimming fluorescent reporter fish. We showcase the value of NeuBtracker for screening neurostimulants with respect to their combined neuronal and behavioral effects and for determining spontaneous and stimulus-induced spatiotemporal patterns of neuronal activation during naturalistic behavior.

PMID:
28967889
DOI:
10.1038/nmeth.4459
[Indexed for MEDLINE]

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