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Mol Cell. 2017 Oct 5;68(1):144-157.e5. doi: 10.1016/j.molcel.2017.09.003. Epub 2017 Sep 28.

P-Body Purification Reveals the Condensation of Repressed mRNA Regulons.

Author information

1
UPMC Univ Paris 06, Institut de Biologie Paris-Seine (IBPS), CNRS UMR 7622, F-75005 Paris, France; Université Côte d'Azur, CNRS, Inserm, iBV, Nice, France. Electronic address: ahubsten@yahoo.fr.
2
UPMC Univ Paris 06, Institut de Biologie Paris-Seine (IBPS), CNRS UMR 7622, F-75005 Paris, France.
3
CNRS UMR-9196, Institut Gustave Roussy, F-94800 Villejuif, France.
4
UPMC Univ Paris 06, Institut de Biologie Paris-Seine (IBPS), CNRS UMR 7622, F-75005 Paris, France; IGMM, CNRS, University Montpellier, F-34090 Montpellier, France.
5
UPMC Univ Paris 06, LPTMC, F-75005 Paris, France.
6
UPMC Univ Paris 06, LUMIC, UMS30, F-75005 Paris, France.
7
UPMC Univ Paris 06, ICAN, F-75013 Paris, France.
8
École Normale Supérieure, PSL Research University, CNRS, Inserm, Institut de Biologie de l'École Normale Supérieure (IBENS), Genomic Paris Centre, IBENS, F-75005 Paris, France.
9
IGMM, CNRS, University Montpellier, F-34090 Montpellier, France.
10
UPMC Univ Paris 06, Institut de Biologie Paris-Seine (IBPS), CNRS UMR 7622, F-75005 Paris, France. Electronic address: dominique.weil@upmc.fr.

Abstract

Within cells, soluble RNPs can switch states to coassemble and condense into liquid or solid bodies. Although these phase transitions have been reconstituted in vitro, for endogenous bodies the diversity of the components, the specificity of the interaction networks, and the function of the coassemblies remain to be characterized. Here, by developing a fluorescence-activated particle sorting (FAPS) method to purify cytosolic processing bodies (P-bodies) from human epithelial cells, we identified hundreds of proteins and thousands of mRNAs that structure a dense network of interactions, separating P-body from non-P-body RNPs. mRNAs segregating into P-bodies are translationally repressed, but not decayed, and this repression explains part of the poor genome-wide correlation between RNA and protein abundance. P-bodies condense thousands of mRNAs that strikingly encode regulatory processes. Thus, we uncovered how P-bodies, by condensing and segregating repressed mRNAs, provide a physical substrate for the coordinated regulation of posttranscriptional mRNA regulons.

KEYWORDS:

P-bodies; RNP condensation; RNP granules; decay; gene expression regulation; phase separation; phase transition; regulon; stress granules; translation repression

PMID:
28965817
DOI:
10.1016/j.molcel.2017.09.003
[Indexed for MEDLINE]
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