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Trends Biochem Sci. 2017 Nov;42(11):850-861. doi: 10.1016/j.tibs.2017.09.001. Epub 2017 Sep 27.

Sharing the SAGA.

Author information

1
CRBM, CNRS, Université de Montpellier, 34293 Montpellier, France. Electronic address: dhelmlinger@crbm.cnrs.fr.
2
Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch, France; Centre National de la Recherche Scientifique, UMR7104, 67404 Illkirch, France; Institut National de la Santé et de la Recherche Médicale, U964, 67404 Illkirch, France; Université de Strasbourg, 67404 Illkirch, France. Electronic address: laszlo@igbmc.fr.

Abstract

Transcription initiation is a major regulatory step in eukaryotic gene expression. Co-activators establish transcriptionally competent promoter architectures and chromatin signatures to allow the formation of the pre-initiation complex (PIC), comprising RNA polymerase II (Pol II) and general transcription factors (GTFs). Many GTFs and co-activators are multisubunit complexes, in which individual components are organized into functional modules carrying specific activities. Recent advances in affinity purification and mass spectrometry analyses have revealed that these complexes often share functional modules, rather than containing unique components. This observation appears remarkably prevalent for chromatin-modifying and remodeling complexes. Here, we use the modular organization of the evolutionary conserved Spt-Ada-Gcn5 acetyltransferase (SAGA) complex as a paradigm to illustrate how co-activators share and combine a relatively limited set of functional tools.

KEYWORDS:

assembly; chromatin; functional module; multisubunit complex; protein–protein interaction; transcription

PMID:
28964624
PMCID:
PMC5660625
DOI:
10.1016/j.tibs.2017.09.001
[Indexed for MEDLINE]
Free PMC Article

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