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Nat Ecol Evol. 2017 Nov;1(11):1747-1756. doi: 10.1038/s41559-017-0314-4. Epub 2017 Sep 25.

Genomic adaptation to polyphagy and insecticides in a major East Asian noctuid pest.

Author information

1
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, China.
2
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, 113-8657, Japan.
3
Molecular Genetics, Centre for DNA Fingerprinting and Diagnostics, Hyderabad, 500001, India.
4
Guangzhou Key Laboratory of Insect Development Regulation and Application Research, School of Life Science, South China Normal University, Guangzhou, 510631, China.
5
Department of Bioscience and Biotechnology, Kyushu University, Fukuoka, 812-8581, Japan.
6
BGI-Shenzhen, Shenzhen, 518083, China.
7
Université Nice Côte d'Azur, INRA, CNRS, ISA, 06903, Sophia Antipolis, France.
8
Sorbonne Universités-UPMC Univ Paris 06, Institute of Ecology & Environmental Sciences of Paris, 75005, Paris, France.
9
INRA, UMR 1392, Institute of Ecology & Environmental Sciences of Paris, 78026, Versailles, France.
10
Laboratoire DGIMI, INRA, Université de Montpellier, 34095, Montpellier, France.
11
Department of Zoology, University of Delhi, Delhi, 110007, India.
12
International Centre for Genetic Engineering and Biotechnology, New Delhi, 110 067, India.
13
Institute of Agrobiological Sciences, NARO, Ibaraki, 305-8634, Japan.
14
Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand.
15
Department of Crop Protection, Ghent University, 9000, Ghent, Belgium.
16
College of Plant Protection and Academy of Agricultural Sciences, Southwest University, Chongqing, 400716, China.
17
Molecular Genetics, Centre for DNA Fingerprinting and Diagnostics, Hyderabad, 500001, India. arun@cdfd.org.in.
18
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, 113-8657, Japan. kishino@lbm.ab.a.u-tokyo.ac.jp.
19
Biological Sciences Department, University of Rhode Island, Kingston, RI, 02881, USA.
20
Guangzhou Key Laboratory of Insect Development Regulation and Application Research, School of Life Science, South China Normal University, Guangzhou, 510631, China. qlfeng@scnu.edu.cn.
21
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, China. xiaqy@swu.edu.cn.
22
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400716, China. mitakazuei@gmail.com.

Abstract

The tobacco cutworm, Spodoptera litura, is among the most widespread and destructive agricultural pests, feeding on over 100 crops throughout tropical and subtropical Asia. By genome sequencing, physical mapping and transcriptome analysis, we found that the gene families encoding receptors for bitter or toxic substances and detoxification enzymes, such as cytochrome P450, carboxylesterase and glutathione-S-transferase, were massively expanded in this polyphagous species, enabling its extraordinary ability to detect and detoxify many plant secondary compounds. Larval exposure to insecticidal toxins induced expression of detoxification genes, and knockdown of representative genes using short interfering RNA (siRNA) reduced larval survival, consistent with their contribution to the insect's natural pesticide tolerance. A population genetics study indicated that this species expanded throughout southeast Asia by migrating along a South India-South China-Japan axis, adapting to wide-ranging ecological conditions with diverse host plants and insecticides, surviving and adapting with the aid of its expanded detoxification systems. The findings of this study will enable the development of new pest management strategies for the control of major agricultural pests such as S. litura.

PMID:
28963452
DOI:
10.1038/s41559-017-0314-4
[Indexed for MEDLINE]

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