Format

Send to

Choose Destination
G3 (Bethesda). 2017 Nov 6;7(11):3831-3836. doi: 10.1534/g3.117.300107.

First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (Formerly Scedosporium prolificans).

Author information

1
Center for Computational Biology, McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
2
Department of Computer Science, Johns Hopkins University, Baltimore, Maryland 21218.
3
Institute for Physical Sciences and Technology, University of Maryland, College Park, Maryland 20742.
4
Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
5
Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205.
6
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287.
7
Center for Computational Biology, McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 szhang28@jhmi.edu salzberg@jhu.edu.
8
Microbiology Laboratory, Johns Hopkins Hospital, Baltimore, Maryland 21287.
9
Department of Biostatistics, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205.

Abstract

Here we describe the sequencing and assembly of the pathogenic fungus Lomentospora prolificans using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. The resulting assembly is highly contiguous, containing a total of 37,627,092 bp with over 98% of the sequence in just 26 scaffolds. Annotation identified 8896 protein-coding genes. Pulsed-field gel analysis suggests that this organism contains at least 7 and possibly 11 chromosomes, the two longest of which have sizes corresponding closely to the sizes of the longest scaffolds, at 6.6 and 5.7 Mb.

KEYWORDS:

fungal genomics; genome assembly; nanopore sequencing; pathogen genomics

PMID:
28963165
PMCID:
PMC5677167
DOI:
10.1534/g3.117.300107
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center