Format

Send to

Choose Destination
Int J Biol Macromol. 2018 Feb;107(Pt A):803-807. doi: 10.1016/j.ijbiomac.2017.09.050. Epub 2017 Sep 28.

In vitro and in vivo anti-inflammatory activities of high molecular weight sulfated polysaccharide; containing fucose separated from Sargassum horneri: Short communication.

Author information

1
Laboratory of Marine Bioresource Technology, Department of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, Republic of Korea.
2
Department of Marine Bio Food Science, Chonnam National University, Yeosu 59626, Republic of Korea.
3
Department of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju 63243, Republic of Korea.
4
Laboratory of Marine Bioresource Technology, Department of Marine Life Science, School of Marine Biomedical Sciences, Jeju National University, Jeju 63243, Republic of Korea.. Electronic address: youjinj@jejunu.ac.kr.

Abstract

Recent studies on crude and pure compounds from Sargassum horneri have shown promising bioactive properties. However, anti-inflammatory potentials of fucose-rich sulfated polysaccharides from S. horneri have not yet been discovered. In the present study, we evaluated the in vitro and in vivo anti-inflammatory activities of four polysaccharide fractions separated from membrane filters according to their molecular weights (<5kDa (f1), 5-10kDa (f2), 10-30kDa (f3), and >30kDa (f4)). According to the results, F4 fraction inhibited the lipopolysaccharides (LPS)-induced nitric oxide (NO) (IC50=87.12μg/mL) and prostaglandin E2 production as well as pro-inflammatory cytokine production in RAW 264.7 cells through down-regulating nuclear factor-κB signaling cascade. According to the results, f4 has a potential to down-regulate LPS-induced toxicity, cell death and NO production levels in LPS-induced in vivo zebrafish embryo model. These results suggest that f4 fraction has the potential to develop functional materials or drugs to treat inflammatory diseases.

KEYWORDS:

Anti-inflammation; Sargassum horneri; Sulfated polysaccharide; Zebrafish

PMID:
28962845
DOI:
10.1016/j.ijbiomac.2017.09.050
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center