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Carbohydr Polym. 2017 Dec 1;177:284-296. doi: 10.1016/j.carbpol.2017.08.130. Epub 2017 Sep 1.

In vitro and in vivo evaluation of effectiveness of a novel TEMPO-oxidized cellulose nanofiber-silk fibroin scaffold in wound healing.

Author information

1
Department of Regenerative Medicine, College of Medicine, Soonchunhyang University 366-1, Ssangyong-dong, Cheonan-City, ChungCheongNam-Do 330-090, Republic of Korea.
2
Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University 366-1, Ssangyong-dong, Cheonan-City, ChungCheongNam-Do 330-090, Republic of Korea.
3
Department of Surgery, College of Medicine, Soonchunhyang University Hospital, Cheonan-City, ChungCheongNam-Do 330-090, Republic of Korea.
4
Department of Plastic and Reconstructive Surgery, College of Medicine, Soonchunhyang University Hospital, Cheonan-City, ChungCheongNam-Do 330-090, Republic of Korea.
5
Division of Environmental Material Engineering, Department of Forest Products, Korea Forest Research Institute, Republic of Korea.
6
Department of Regenerative Medicine, College of Medicine, Soonchunhyang University 366-1, Ssangyong-dong, Cheonan-City, ChungCheongNam-Do 330-090, Republic of Korea; Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University 366-1, Ssangyong-dong, Cheonan-City, ChungCheongNam-Do 330-090, Republic of Korea. Electronic address: lbt@sch.ac.kr.

Abstract

In this study, a novel TEMPO-oxidized cellulose nanofiber (TOCN)-silk fibroin scaffold was prepared using a cost effective freeze drying method. Fundamental physical characterizations were carried out by scanning electron microscopy (SEM), pore diameter determination, FT-IR. PBS uptake behavior of the scaffold showed that, silk fibroin can enhance the swelling capacity of TOCN. L929 primary fibroblast cell was selected for in vitro studies, which showed that the scaffolds facilitated growth of cells. In vivo evaluation of TOCN, TOCN-silk fibroin composites was examined using critical sized rat skin excisional model for one and two weeks. The results of rat wound model revealed that, compared to only TOCN scaffold, TOCN-silk fibroin scaffold successfully promoted wound healing by the expression of wound healing markers. TOCN-silk fibroin 2% has the fastest wound healing capacity. Thus, it appears that TOCN-silk fibroin composite scaffolds can be useful as wound healing material in clinical applications.

KEYWORDS:

Excisional wound; Oxidized cellulose nano-fiber; Silk fibroin; Wound healing

PMID:
28962770
DOI:
10.1016/j.carbpol.2017.08.130
[Indexed for MEDLINE]

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