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Int J Environ Res Public Health. 2017 Sep 29;14(10). pii: E1149. doi: 10.3390/ijerph14101149.

A Marine Bacterium, Bacillus sp. Isolated from the Sediment Samples of Algoa Bay in South Africa Produces a Polysaccharide-Bioflocculant.

Author information

1
SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa. nntozonke@ufh.ac.za.
2
Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa. nntozonke@ufh.ac.za.
3
SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa. kokaiyeto@ufh.ac.za.
4
Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa. kokaiyeto@ufh.ac.za.
5
GenØK-Centre for Biosafety, Forskningsparken i Breivika, Postboks 6418, 9294 Tromsø, Norway. aso023@post.uit.no.
6
Department of Microbiology, School of Life Sciences, University of KwaZulu-Natal, Private Bag X54001, Durban 4000, South Africa. olanirana@ukzn.ac.za.
7
SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa. unwodo@ufh.ac.za.
8
Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa. unwodo@ufh.ac.za.
9
SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa. aokoh@ufh.ac.za.
10
Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa. aokoh@ufh.ac.za.

Abstract

Bioflocculants mediate the removal of suspended particles from solution and the efficiency of flocculation is dependent on the characteristics of the flocculant. Apart from the merits of biodegradability and harmlessness, bioflocculants could be viable as industrially relevant flocculants as they are a renewable resource. Additionally, the shortcomings associated with the conventionally used flocculants such as aluminium salts and acrylamide polymers, which include dementia and cancer, highlight more the need to use bioflocculants as an alternative. Consequently, in this study a marine sediment bacterial isolate was screened for bioflocculant production. Basic local alignment search tools (BLAST) analysis of 16S ribosomal deoxyribonucleic acid (rDNA) sequence of the bacterial isolate showed 98% similarity to Bacillus thuringiensis MR-R1. The bacteria produced bioflocculant optimally with inoculum size (4% v/v) (85%), glucose (85.65%) and mixed nitrogen source (urea, ammonium chloride and yeast extract) (75.9%) and the divalent cation (Ca2+) (62.3%). Under optimal conditions, a maximum flocculating activity of over 85% was attained after 60 h of cultivation. The purified polysaccharide-bioflocculant flocculated optimally at alkaline pH 12 (81%), in the presence of Mn2+ (73%) and Ca2+ (72.8%). The high flocculation activity shown indicates that the bioflocculant may contend favourably as an alternative to the conventionally used flocculants in water treatment.

KEYWORDS:

Bacillus sp.; bioflocculants; flocculation; marine environment; polysaccharide

PMID:
28961180
PMCID:
PMC5664650
DOI:
10.3390/ijerph14101149
[Indexed for MEDLINE]
Free PMC Article

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