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Int J Mol Sci. 2017 Sep 29;18(10). pii: E2073. doi: 10.3390/ijms18102073.

High Levels of Circulating Type II Collagen Degradation Marker (CTx-II) Are Associated with Specific VDR Polymorphisms in Patients with Adult Vertebral Osteochondrosis.

Author information

1
Department of Medicine, University of Udine, 33100 Udine, Italy. sabina.cauci@uniud.it.
2
Orthopaedic Biotechnology Lab, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161 Milan, Italy. marco.vigano@grupposandonato.it.
3
Orthopaedic Biotechnology Lab, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161 Milan, Italy. laura.degirolamo@grupposandonato.it.
4
Orthopaedic Biotechnology Lab, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161 Milan, Italy. deluca.paola@grupposandonato.it.
5
Orthopaedic Biotechnology Lab, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161 Milan, Italy. carlotta.perucca@grupposandonato.it.
6
Vita-Salute San Raffaele University, 20132 Milan, Italy. banfi.giuseppe@fondazionesanraffaele.it.
7
Laboratory of Experimental Biochemistry & Molecular Biology, IRCCS Galeazzi Orthopaedic Institute, 20161 Milan, Italy. giovanni.lombardi@grupposandonato.it.
8
Department of Orthopedics and Traumatology, Spine Surgery III-Scoliosis Unit, IRCCS Galeazzi Orthopaedic Institute, 20161 Milan, Italy. marco.brayda@spinecaregroup.it.
9
Orthopaedic Biotechnology Lab, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161 Milan, Italy. alessandra.colombini@grupposandonato.it.

Abstract

Both vitamin D and collagen have roles in osteocartilaginous homeostasis. We evaluated the association between the circulating 25-hydroxyvitamin D (25(OH)D) type I and II collagen degradation products (CTx-I, and CTx-II), and four vitamin D receptor gene (VDR) polymorphisms, in Italian males affected by low back pain (LBP) due to herniation/discopathy and/or vertebral osteochondrosis. FokI, BsmI, ApaI, and TaqI VDR-polymorphisms were detected through PCR-restriction fragment length polymorphism (RFLP), and circulating 25(OH)D, CTx-I and CTx-II were measured by immunoassays in 79 patients (of which 26 had osteochondrosis) and 79 age-, sex- and body mass index (BMI)-matched healthy controls. Among all 158 subjects, carriers of FF and Ff genotypes showed lower 25(OH)D than ff, which suggested a higher depletion of vitamin D in F allele carriers. Higher CTx-I concentrations were observed in TT versus Tt among controls, and Tt versus tt among LBP cases, which suggested a higher bone-cartilaginous catabolism in subjects bearing the T allele. Higher CTx-II concentrations were observed in patients with osteochondrosis bearing FF, bb, TT, or Aa genotypes in comparison with hernia/discopathy patients and healthy controls. Vertebral osteochondrosis shows peculiar genotypic and biochemical features related to vitamin D and the osteocartilaginous metabolism. Vitamin D has roles in the pathophysiology of osteochondrosis.

KEYWORDS:

Italian males; VDR polymorphisms; adult vertebral osteochondrosis; type II collagen

PMID:
28961166
PMCID:
PMC5666755
DOI:
10.3390/ijms18102073
[Indexed for MEDLINE]
Free PMC Article

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