Format

Send to

Choose Destination
Adv Mater. 2017 Nov;29(42). doi: 10.1002/adma.201703489. Epub 2017 Sep 28.

Solvent-Assisted Micromolding of Biohybrid Hydrogels to Maintain Human Hematopoietic Stem and Progenitor Cells Ex Vivo.

Author information

1
Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials Dresden, Dresden, Germany.
2
Institute of Biochemistry, Universität Leipzig, Leipzig, Germany.
3
Center for Regenerative Therapies Dresden, Technische Universität Dresden, Dresden, Germany.

Abstract

Array-format cell-culture carriers providing tunable matrix cues are instrumental in current cell biology and bioengineering. A new solvent-assisted demolding approach for the fabrication of microcavity arrays with very small feature sizes down to single-cell level (3 µm) of very soft biohybrid glycosaminoglycan-poly(ethylene glycol) hydrogels (down to a shear modulus of 1 kPa) is reported. It is further shown that independent additional options of localized conjugation of adhesion ligand peptides, presentation of growth factors through complexation to gel-based glycosaminoglycans, and secondary gel deposition for 3D cell embedding enable a versatile customization of the hydrogel microcavity arrays for cell culture studies. As a proof of concept, cell-instructive hydrogel compartment arrays are used to analyze the response of human hematopoietic stem and progenitor cells to defined biomolecular and spatial cues.

KEYWORDS:

biohybrids; hematopoietic stem cells; hydrogels; microcavities; micromolding

PMID:
28960524
DOI:
10.1002/adma.201703489

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center