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Chem Commun (Camb). 2017 Oct 10;53(81):11245-11248. doi: 10.1039/c7cc06004a.

Impact of cellular health conditions on the protein folding state in mammalian cells.

Author information

1
RIKEN Quantitative Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan. kinomata@riken.jp kigawa@riken.jp and PRESTO/Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
2
Department of Chemistry, Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo 192-0397, Japan.
3
RIKEN Quantitative Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan. kinomata@riken.jp kigawa@riken.jp.
4
RIKEN Quantitative Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan. kinomata@riken.jp kigawa@riken.jp and Department of Computer Science, School of Computing, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8503, Japan.

Abstract

By using in-cell NMR experiments, we have demonstrated that the protein folding state in cells is significantly influenced by the cellular health conditions. hAK1 was denatured in cells under stressful culture conditions, while it remained functional and properly folded in cells continuously supplied with a fresh medium.

PMID:
28960222
DOI:
10.1039/c7cc06004a
[Indexed for MEDLINE]

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