Format

Send to

Choose Destination
Nanomedicine (Lond). 2017 Nov;12(21):2597-2609. doi: 10.2217/nnm-2017-0161. Epub 2017 Sep 29.

Nanofiber-based sutures induce endogenous antimicrobial peptide.

Author information

1
Department of Surgery-Transplant & Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE 68198, USA.
2
Chongqing Academy of Animal Sciences & Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Chongqing, China.
3
Department of Biochemistry & Biophysics & Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA.
4
Department of Orthopedic Surgery, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25755, USA.
5
Department of Surgery-General Surgery, University of Nebraska Medical Center, Omaha, NE 68198, USA.
6
Department of Surgery-Plastic Surgery, University of Nebraska Medical Center, Omaha, NE 68198, USA.

Abstract

AIM:

The aim of this study was to develop nanofiber-based sutures capable of inducing endogenous antimicrobial peptide production.

METHODS:

We used co-axial electrospinning deposition and rolling to fabricate sutures containing pam3CSK4 peptide and 25-hydroxyvitamin D3 (25D3).

RESULTS:

The diameters and mechanical properties of the sutures were adjustable to meet the criteria of United States Pharmacopeia designation. 25D3 exhibited a sustained release from nanofiber sutures over 4 weeks. Pam3CSK4 peptide also showed an initial burst followed by a sustained release over 4 weeks. The co-delivery of 25D3 and pam3CSK4 peptide enhanced cathelicidin antimicrobial peptide production from U937 cells and keratinocytes compared with 25D3 delivery alone. In addition, the 25D3/pam3CSK4 peptide co-loaded nanofiber sutures did not significantly influence proliferation of keratinocytes, fibroblasts, or the monocytic cell lines U937 and HL-60.

CONCLUSION:

The use of 25D3/pam3CSK4 peptide co-loaded nanofiber sutures could potentially induce endogenous antimicrobial peptide production and reduce surgical site infections.

KEYWORDS:

25-hydroxyvitamin D3; co-delivery; electrospun nanofibers; pam3Cys-Ser-(Lys)4 peptide; surgical site infection; sutures

PMID:
28960168
PMCID:
PMC5674207
DOI:
10.2217/nnm-2017-0161
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Atypon Icon for PubMed Central
Loading ...
Support Center