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Zhongguo Zhong Yao Za Zhi. 2017 Feb;42(4):675-679. doi: 10.19540/j.cnki.cjcmm.20170103.024.

[Analysis of transicriptomes and exploring flavonoid biosynthetic pathway genes in Lithocarpus polystachyus].

[Article in Chinese]

Author information

1
College of Life Sciences, North China University of Science and Technology, Tangshan 063000, China.
2
College of Agricultural and Food Engineering, Baise University, Baise 533000, China.

Abstract

The sweet taste and health effect of Lithocarpus polystachyus are mainly related flavonoid. To obtain Lithocarpus transcriptome database and flavonoid biosynthesis-related genes, the RNA-Seq techology (Illumina HiSeq 4000) was used to sequence its transcriptome. Six Gb database was assembled after assembly steps, and 41 043 of L. polystachyus unigenes were obtained. With blasting them with 7 data banks, all unigenes were involved in 51 GO-terms and 237 metabolic pathways. And furthermore 28 genes of the flavonoid biosynthesis-related were found. After using the MicroSatallite, 18 161 SSR were obtained, the single-nucleotide-repeated was the richest at 7 346. These data represent abundant messages about transcripts and provide valuable genome data sources in molecular biology of L. polystachyus.

KEYWORDS:

Lithocarpus polystachyus ; flavonoids; high-throughput sequencing ; transcriptome

[Indexed for MEDLINE]

Conflict of interest statement

The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose.

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