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Curr Med Mycol. 2016 Dec;2(4):30-36. doi: 10.18869/acadpub.cmm.2.4.30.

In vitro activity of kombucha tea ethyl acetate fraction against Malassezia species isolated from seborrhoeic dermatitis.

Author information

Department of Mycology, Medical School, Alborz University of Medical Sciences, Karaj, Iran.
Department of Dermatology, Medical School, Alborz University of Medical Sciences, Karaj, Iran.
Department of Bacteriology, Medical School, Alborz University of Medical Sciences, Karaj, Iran.
School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
Department of Food Sciences and Technology, Faculty of Agriculture, Islamic Azad University, Shahr-e Kord, Iran.
Department of Pathobiology, Food Microbiology Division, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.



Seborrheic dermatitis is a chronic and recurrent superficial dermatitis in which Malassezia species play an important role. There are different Malassezia species, which have been recently reported to be resistant to common antifungals. Natural sources can be useful alternatives to reduce the emergence of this resistance. Kombucha tea is believed to have potential antimicrobial properties. Regarding this, the present study aimed to investigate the antifungal activity of Kombucha tea ethyl acetate fraction (KEAF) against Malassezia species obtained from the patients with seborrheic dermatitis.


A total of 23 clinical isolates were identified by direct microscopic examination and Tween assimilation, and then confirmed by DNA sequencing of ITS regions for Malassezia species. Kombucha tea was fractionated using ethyl acetate (1:2 v/v). The minimum inhibitory concentration (MIC) microdilution assay was used to evaluate the anti-Malssezia activity of KEAF at three concentrations of 10, 40, and 80 mg/mL.


The results of the DNA sequence analysis indicated that M. furfur (39.13%) was the predominant species, followed by M. globosa (30.43%), M. sloofie (13.04%), M. sympodialis (13.04%), and M. restricta (4.34%), respectively. Furthermore, KEAF showed inhibitory activity against Malassezia species. Accordingly, KEAF had the lowest and highest MIC value against M. sloofie and M. restricta, respectively. Moreover, the inhibitory effect of the extract was equivalent to that of ketoconazole at 4.8 µg/mL.


The findings of the current study highlighted the antifungal properties of KEAF. Therefore, this extract can be promoted as complementary medicine for the treatment of the infections caused by Malassezia.


Antifungal activity; Ethyle acetate fraction; Ketoconazole; Kombucha tea; Malassezia spp

Conflict of interest statement

The authors have declared no conflicts of interest.

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