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J AOAC Int. 2018 May 1;101(3):793-797. doi: 10.5740/jaoacint.17-0252. Epub 2017 Sep 28.

Development and Method Validation of Analysis of Urushiol in Sumac and Food Ingredients in Korea.

Author information

1
Korea Food Research Institute, Food Analysis Center, 62, Anyangpangyo-ro 1201 Beon-gil, Bundang-gu, Seongnam 13539, Republic of Korea; Korea University, Department of Biotechnology, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea.
2
Korea Food Research Institute, Food Analysis Center, 62, Anyangpangyo-ro 1201 Beon-gil, Bundang-gu, Seongnam 13539, Republic of Korea.
3
World Institute of Kimchi, 86 Kimchi-ro, Nam-gu, Gwangju 61755, Republic of Korea.
4
Korea University, Department of Biotechnology, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea.

Abstract

This study developed an analytical method to determine the urushiol content in sap and several foods. The full process for urushiol analysis consists of extraction, trimethylsilyl silylation, analysis, and identification via GC-MS, with each step optimized to attain the required accuracy and precision. Urushiol was separated from sap via liquid-liquid extraction and was derivatized via silylation. The components were analyzed using a polar capillary column and identified using GC-MS. The deviations of relative retention times and retention time windows were within 0.001 and 0.02 min, which satisfied the criteria of 0.06 and 0.03 min, respectively. The response of the urushiol standards tested was found to be linear in the investigated concentration range, with a correlation coefficient of 0.998. The LODs were between 1.74 and 2.67 μg/mL.

PMID:
28958219
DOI:
10.5740/jaoacint.17-0252
[Indexed for MEDLINE]

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