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3 Biotech. 2017 Oct;7(5):321. doi: 10.1007/s13205-017-0965-5. Epub 2017 Sep 16.

Application of customised bacterial inoculants for grass haylage production and its effectiveness on nutrient composition and fermentation quality of haylage.

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Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration, Seonghwan-Eup, Cheonan, Chungnam 31000 Republic of Korea.
Laboratory of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Aoba, Sendai, 980-8577 Japan.
Jungnong Bio Inc, Goksung, Chonnam 57509 Republic of Korea.
Pyung Hwa Dairy Farm, Young Gwang, Chunnam, 57031 Republic of Korea.
Contributed equally


The present study aimed to investigate the efficacy of customised Lactobacillus plantarum KCC-10, KCC-19 and K-46 on nutrient composition and fermentation quality of low moisture Italian ryegrass (IRG) forage. An addition of customised bacterial inoculants (CBI) did not affect the nutrient compositions and digestibility rates of haylage. The lactic acid content was higher in CBI-inoculated haylage, whereas the amount of acetic acid and butyric acid production was significantly reduced than the control. CBI-inoculated haylage exhibited higher numbers of bacterial colonies that reduced the pH of the haylage. Low pH in haylage is an important criterion for preventing undesirable microbial growth and improves fermentation quality of haylage. PCR studies indicated that the DNA of L. plantarum was predominantly amplified. It evidenced that the CBI is the main reason behind the improvement of haylage fermentation as compared to control. Overall results suggested that KCC-10, KCC-19 and K-46 are considered as potent strains for improving fermentation quality of low moisture forage and preserve its stability for a long time.


Fermentation quality; Italian ryegrass; L. plantarum; Low moisture forage; Nutrient and digestibility rates

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