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Reprod Toxicol. 2017 Dec;74:189-194. doi: 10.1016/j.reprotox.2017.09.008. Epub 2017 Sep 22.

Susceptibility of human cumulus cells to bisphenol a In vitro.

Author information

1
Infertility and IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Center and Sackler School of Medicine, Tel-Aviv University, Israel.
2
Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham & Women's Hospital and Harvard Medical School, Boston, MA, USA.
3
Biology Department, Middlebury College, Middlebury, VT, USA.
4
Tamman Cardiovascular ResearchInstitute, Leviev Heart Center, Regenerative Medicine, Stem Cells and Tissue Engineering Center, Chaim Sheba Medical Center, Israel.
5
Infertility and IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Center and Sackler School of Medicine, Tel-Aviv University, Israel. Electronic address: Ronit.Machtinger@sheba.health.gov.il.

Abstract

Bisphenol A (BPA) is detectable in follicular fluid. However, the effect of BPA exposure on human cumulus cells (CC) that surround the oocyte and are crucial for oocyte competence has been largely unexplored. We exposed primary cultures of CC to increasing concentrations of BPA [0,0.002, 0.02 and 20μg/mL] and tested the effects of BPA on the expression of genes associated with apoptosis using quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR); we also assessed the effect of BPA on apoptosis by staining with anti-caspase 3. Exposure to 20μg/mL BPA led to significantly decreased expression of CDC20, BUB1B and HAS2 (p<0.03), increased expression of TRIB3 and LUM (p≤0.005), and increased frequency of cells positive for anti-CASP3 (p=0.03), compared to control. Our results imply that BPA may lead to ovarian toxicity by increasing CC apoptosis and provide an important molecular mechanism for the effect of BPA on human CC in vitro.

KEYWORDS:

Apoptosis; Bisphenol A; Cumulus cells; Human primary cultures

PMID:
28951153
DOI:
10.1016/j.reprotox.2017.09.008
[Indexed for MEDLINE]

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