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Bioresour Technol. 2018 Jan;247:242-249. doi: 10.1016/j.biortech.2017.09.080. Epub 2017 Sep 14.

Ethanol production from bamboo using mild alkaline pre-extraction followed by alkaline hydrogen peroxide pretreatment.

Author information

1
Department of Chemical and Biological Engineering, University of British Columbia, 2360 East Mall, Vancouver, British Columbia V6T 1Z4, Canada.
2
Tianjin Key Laboratory of Pulp & Paper, Tianjin University of Science and Technology, Tianjin 300457, China.
3
Department of Chemical and Biological Engineering, University of British Columbia, 2360 East Mall, Vancouver, British Columbia V6T 1Z4, Canada. Electronic address: nuwan.kapu@ubc.ca.

Abstract

A sequential two-stage pretreatment process comprising alkaline pre-extraction and alkaline hydrogen peroxide pretreatment (AHP) was investigated to convert bamboo carbohydrates into bioethanol. The results showed that mild alkaline pre-extraction using 8% (w/w) sodium hydroxide (NaOH) at 100°C for 180min followed by AHP pretreatment with 4% (w/w) hydrogen peroxide (H2O2) was sufficient to generate a substrate that could be efficiently digested with low enzyme loadings. Moreover, alkali pre-extraction enabled the use of lower H2O2 charges in AHP treatment. Two-stage pretreatment followed by enzymatic hydrolysis with only 9FPU/g cellulose led to the recovery of 87% of the original sugars in the raw feedstock. The use of the pentose-hexose fermenting Saccharomyces cerevisiae SR8u strain enabled the utilization of 95.7% sugars in the hydrolysate to reach 4.6%w/v ethanol titer. The overall process also enabled the recovery of 62.9% lignin and 93.8% silica at high levels of purity.

KEYWORDS:

Alkaline hydrogen peroxide; Alkaline pretreatment; Bamboo; Biorefinery; Enzymatic hydrolysis; Ethanol

PMID:
28950132
DOI:
10.1016/j.biortech.2017.09.080
[Indexed for MEDLINE]

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