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Emerg Infect Dis. 2017 Nov;23(11). doi: 10.3201/eid2311.171131. Epub 2017 Nov 17.

Lineage-Specific Real-Time RT-PCR for Yellow Fever Virus Outbreak Surveillance, Brazil.

Abstract

The current yellow fever outbreak in Brazil prompted widespread yellow fever virus (YFV) vaccination campaigns, imposing a responsibility to distinguish between vaccine- and wild-type YFV-associated disease. We developed novel multiplex real-time reverse transcription PCRs that differentiate between vaccine and American wild-type YFV. We validated these highly specific and sensitive assays in an outbreak setting.

KEYWORDS:

Americas; Brazil; real-time RT-PCR; reverse transcription PCR; surveillance; vaccine safety; vector-borne infections; viruses; yellow fever virus

PMID:
28949285
PMCID:
PMC5652426
DOI:
10.3201/eid2311.171131
[Indexed for MEDLINE]
Free PMC Article

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