Send to

Choose Destination
J Pept Sci. 2017 Nov;23(11):833-839. doi: 10.1002/psc.3046. Epub 2017 Sep 26.

The American cockroach peptide periplanetasin-4 inhibits Clostridium difficile toxin A-induced cell toxicities and inflammatory responses in the mouse gut.

Author information

Division of Life Science and Chemistry, College of Natural Science, Daejin University, Pocheon, Gyeonggido, 11159, Korea.
Hainan Institute of Science and Technology, Haikou, 571126, China.
Lee Gil Ya Cancer and Diabetes Institute, Gachon University Graduate School of Medicine, Incheon, 406-840, Korea.
Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju, 55365, Korea.


Many reports have shown that crude extracts of the American cockroach have therapeutic effects on inflammation. In a previous study, our research group showed that an antimicrobial peptide (Periplanetasin-2) derived from the American cockroach via de novo transcriptome analysis inhibited apoptosis of human colonocytes and inflammatory responses of the mouse gut caused by Clostridium difficile toxin A. Here, we examined whether Periplanetasin-4 (Peri-4), another antimicrobial peptide identified via de novo transcriptome analysis of the American cockroach, could also inhibit the various toxicities induced by C. difficile toxin A. We found that Peri-4 significantly reduced the cell viability loss and cell apoptosis caused by toxin A in vitro. Peri-4 also ameliorated the severe inflammatory responses seen in the toxin A-induced mouse enteritis model, rescuing the villus disruption and interleukin-6 production induced by luminal injection of toxin A into the mouse gut. Mechanistically, we found that Peri-4 could reduce toxin A-induced reactive oxygen species production to inhibit the activations of p38MAPK and p21Cip1/Waf1 , which are critical for the cell damages induced by toxin A. These results collectively suggest that the Peri-4 may be a potential therapeutic agent for treating toxin A-induced pseudomembranous colitis.


American cockroach peptide; Clostridium difficile; anti-inflammation; colonocytes; gut; toxin A

[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center