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ACS Synth Biol. 2018 Jan 19;7(1):166-175. doi: 10.1021/acssynbio.7b00266. Epub 2017 Oct 30.

A Modular Receptor Platform To Expand the Sensing Repertoire of Bacteria.

Author information

1
Centre de Biochimie Structurale, INSERM U1054, CNRS UMR5048, University of Montpellier , 34090 Montpellier, France.
2
Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay , 91191 Gif-sur-Yvette, France.

Abstract

Engineered bacteria promise to revolutionize diagnostics and therapeutics, yet many applications are precluded by the limited number of detectable signals. Here we present a general framework to engineer synthetic receptors enabling bacterial cells to respond to novel ligands. These receptors are activated via ligand-induced dimerization of a single-domain antibody fused to monomeric DNA-binding domains (split-DBDs). Using E. coli as a model system, we engineer both transmembrane and cytosolic receptors using a VHH for ligand detection and demonstrate the scalability of our platform by using the DBDs of two different transcriptional regulators. We provide a method to optimize receptor behavior by finely tuning protein expression levels and optimizing interdomain linker regions. Finally, we show that these receptors can be connected to downstream synthetic gene circuits for further signal processing. The general nature of the split-DBD principle and the versatility of antibody-based detection should support the deployment of these receptors into various hosts to detect ligands for which no receptor is found in nature.

PMID:
28946740
PMCID:
PMC5880506
DOI:
10.1021/acssynbio.7b00266
[Indexed for MEDLINE]
Free PMC Article

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