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J Med Food. 2017 Nov;20(11):1063-1067. doi: 10.1089/jmf.2017.0063. Epub 2017 Sep 25.

Antiproliferative Activity of Walnut (Juglans regia L.) Proteins and Walnut Protein Hydrolysates.

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1 Laboratory of Functional Foods, Faculty of Foods Science and Engineering, Technical University of Ambato , Ambato, Ecuador .
2 Research Department Faculty of Health and Human Sciences, Bolivar State University , Guaranda, Ecuador .
3 Research Institute of Food Science (CIAL), (CSIC-UAM), Cantoblanco Campus, Autonomous University of Madrid, Madrid, Spain .
4 CPQBA, State University of Campinas (UNICAMP) , Paulínia, Brazil .


Proteins from Juglans regia L. were isolated. Then, proteins were hydrolyzed with different enzymes. Antiproliferative activity of proteins and of the protein hydrolysates of J. regia L. were evaluated using the sulforhodamine B method. Glutelin and prolamin proteins presented a high antiproliferative activity against cancer cells PC-3 (prostate) and K-562 (leukemia) with values of 43.9 and 84.4 μg/mL, respectively. The highest inhibitory effect observed was 50% at 0.25 μg/mL concentration in gastrointestinal digestion with pepsin and corolase pp in a dose-dependent manner against cancer cell UACC62 (melanoma). Pepsin hydrolysate showed inhibitory effects against cancer cell UACC62 (melanoma) with a concentration of 71.0 μg/mL. The effects were studied in a dose-dependent manner. The hydrolysate obtained with neutrase enzyme presented inhibitory effects against cancer cell UACC62 (melanoma) at a concentration of 25 μg/mL. Neither proteins nor protein hydrolysates presented cytotoxicity against normal cell assay VERO (epithelial).


antiproliferative activity; cytotoxicity; hydrolysates; walnut proteins

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