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Comp Biochem Physiol C Toxicol Pharmacol. 2018 Jun;208:105-113. doi: 10.1016/j.cbpc.2017.09.007. Epub 2017 Sep 21.

Assaying uptake of endocrine disruptor compounds in zebrafish embryos and larvae.

Author information

1
University of Alabama at Birmingham, Department of Pharmacology and Toxicology, Birmingham, AL 35294, USA. Electronic address: jsouder@uab.edu.
2
University of Alabama at Birmingham, Department of Pharmacology and Toxicology, Birmingham, AL 35294, USA. Electronic address: danielg@uab.edu.

Abstract

To study the effects of environmental endocrine disruptor compounds (EDCs) on aquatic animals, embryos and larvae are typically incubated in water containing defined concentrations of EDCs. However, the amount of EDC uptake into the animal is often difficult to determine. Using radiolabeled estradiol ([3H]E2), we previously developed a rapid, straightforward assay to measure estradiol uptake from water into zebrafish embryos and larvae. Here, we extend this approach to measure the uptake of two additional EDCs, bisphenol A (BPA) and ethinyl estradiol (EE2). As with E2, the uptake of each compound by individual larvae was low (<6%), and increased with increasing concentration, duration, and developmental stage. We found that E2 and EE2 had similar uptake under equivalent exposure conditions, while BPA had comparatively lower uptake. One application of this assay is to test factors that influence EDC uptake or efflux. It has been suggested that persistent organic pollutants (POPs) inhibit ABC transporters that may normally efflux EDCs and their metabolites, inducing toxicity in aquatic organisms. We measured [3H]E2 levels in zebrafish in the presence or absence of the POP PDBE-100, and cyclosporine A, a known inhibitor of ABC transporters. Neither chemical significantly affected [3H]E2 levels in zebrafish, suggesting that zebrafish maintain estradiol efflux in the presence of PDBE-100, independently of cyclosporine A-responsive transporters. These uptake results will be a valuable reference for EDC exposure studies in developing zebrafish, and provide a rapid assay to screen for chemicals that influence estrogen-like EDC levels in vivo.

KEYWORDS:

Bisphenol A; Endocrine disruptors; Estrogens; Zebrafish

PMID:
28943455
PMCID:
PMC5862746
[Available on 2019-06-01]
DOI:
10.1016/j.cbpc.2017.09.007
[Indexed for MEDLINE]

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