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Clin Immunol. 2017 Oct;183:325-335. doi: 10.1016/j.clim.2017.09.019. Epub 2017 Sep 22.

Standardized whole blood stimulation improves immunomonitoring of induced immune responses in multi-center study.

Author information

1
Center for Translational Research, Institut Pasteur, Paris, France; Immunobiology of Dendritic Cells, Institut Pasteur, Paris, France; INSERM U1223, Institut Pasteur, Paris, France. Electronic address: darragh.duffy@pasteur.fr.
2
Center for Translational Research, Institut Pasteur, Paris, France.
3
CHU Nantes, Laboratoire d'Immunologie, CIMNA, Nantes, France; Centre de Recherche en Transplantation et Immunologie UMR 1064, Inserm, Université de Nantes, Nantes, France.
4
Laboratory of Vaccinology and Mucosal Immunity, Université Libre de Bruxelles (ULB), Brussels, Belgium.
5
Center for Translational Research, Institut Pasteur, Paris, France; ICAReB Platform, Center for Translational Research, Institut Pasteur, Paris, France.
6
CHU Nantes, Laboratoire d'Immunologie, CIMNA, Nantes, France; Centre de Recherche en Transplantation et Immunologie UMR 1064, Inserm, Université de Nantes, Nantes, France; LabEx IGO Immunotherapy Graf-Oncology, Nantes, France.
7
Myriad RBM Inc, Austin, TX, USA.
8
Laboratoire d'Immunologie clinique, CIC-4218 et Unité Inserm 932 Institut Curie, Paris, France.
9
Germans Trias i Pujol Hospital, Dept Cellular Biology, Physiology, Immunology, UAB, Barcelona, Spain.
10
Laboratory of Vaccinology and Mucosal Immunity, Université Libre de Bruxelles (ULB), Brussels, Belgium; Immunobiology Clinic, Hôpital Erasme, Université Libre de Bruxelles (ULB), Brussels, Belgium.
11
Complejo Asistencial Universitario de León, Leon, Spain.
12
INSERM UMR1160, Université Paris Diderot, AP-HP, Hopital St Louis, Paris, France.
13
HOT Screen GmbH, Reutlingen, Germany.
14
Center for Translational Research, Institut Pasteur, Paris, France; Immunobiology of Dendritic Cells, Institut Pasteur, Paris, France; INSERM U1223, Institut Pasteur, Paris, France; Department of Cancer Immunology, Genentech Inc., San Francisco, CA 94080, USA. Electronic address: albert.matthew@gene.com.

Abstract

Functional immune responses are increasingly important for clinical studies, providing in depth biomarker information to assess immunotherapy or vaccination. Incorporating functional immune assays into routine clinical practice has remained limited due to challenges in standardizing sample preparation. We recently described the use of a whole blood syringe-based system, TruCulture®, which permits point-of-care standardized immune stimulation. Here, we report on a multi-center clinical study in seven FOCIS Centers of Excellence to directly compare TruCulture to conventional PBMC methods. Whole blood and PBMCs from healthy donors were exposed to LPS, anti-CD3 anti-CD28 antibodies, or media alone. 55 protein analytes were analyzed centrally by Luminex multi-analyte profiling in a CLIA-certified laboratory. TruCulture responses showed greater reproducibility and improved the statistical power for monitoring differential immune response activation. The use of TruCulture addresses a major unmet need through a robust and flexible method for immunomonitoring that can be reproducibly applied in multi-center clinical studies.

ONE SENTENCE SUMMARY:

A multi-center study revealed greater reproducibility from whole blood stimulation systems as compared to PBMC stimulation for studying induced immune responses.

KEYWORDS:

Cytokines; Functional immune responses; Immunomonitoring; Multi-center clinical studies; PBMCs; Whole blood

PMID:
28943400
DOI:
10.1016/j.clim.2017.09.019
[Indexed for MEDLINE]

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