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Dev Comp Immunol. 2018 Jan;78:91-99. doi: 10.1016/j.dci.2017.09.016. Epub 2017 Sep 21.

Identification and characterization of a rabbit novel IFN-α unlocated in genome.

Author information

1
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, PR China.
2
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, PR China.
3
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Jilin University, Jilin, Changchun 130062, PR China; Jiangsu Coinnovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, PR China. Electronic address: hanwy@jlu.edu.cn.

Abstract

The multigene family of rabbit IFN-α (RbIFN-α) is located on chromosome 1, which shows seven functional genes in type I IFN locus. A novel RbIFN-α that remains unlocated in the rabbit genome was amplified and designated as the first novel rabbit IFN-α (RbIFN-αNov1), which possesses the typical molecular characteristics of type I IFNs and could be induced in RK-13 cells and peripheral blood mononuclear cells. After the mature peptide of RbIFN-αNov1 was expressed, its antiviral activity, physicochemical characteristics, and cytotoxicity were determined in vitro. Results indicated that RbIFN-αNov1 exerted a high specific antiviral activity against VSV and a low cytotoxic effect on RK-13 cells. RbIFN-αNov1 showed high sensitivity to trypsin and remained relatively stable after acid, alkali, or heat treatment. RbIFN-αNov1 could induce Mx1 expression on RK-13 cells and activate the NF-κB, ISRE and BoIFN-β promoter activities on bovine testicular cells. Overall, our research on RbIFN-αNov1 not only enriches the knowledge about rabbit IFNs but also makes RbIFN-αNov1 have the potential to be used as an effective therapeutic agent for rabbit viral diseases.

KEYWORDS:

Biological activity; Expression distribution; Molecular characteristics; Rabbit interferon-α; Type I IFN locus

PMID:
28942155
DOI:
10.1016/j.dci.2017.09.016
[Indexed for MEDLINE]

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