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Clin Chem. 2017 Nov;63(11):1745-1752. doi: 10.1373/clinchem.2017.275578. Epub 2017 Sep 22.

Rapid, Secure Drug Testing Using Fingerprint Development and Paper Spray Mass Spectrometry.

Author information

1
Ion Beam Centre, University of Surrey, Surrey, UK; c.d.costa@surrey.ac.uk.
2
Ion Beam Centre, University of Surrey, Surrey, UK.
3
University of Surrey, Department of Chemistry, Surrey, UK.
4
Netherlands Forensic Institute, the Hague, the Netherlands.
5
Department of Chemical Engineering, Delft University of Technology, Delft, the Netherlands.
6
Intelligent Fingerprinting, Cambridge, UK.

Abstract

BACKGROUND:

Paper spray mass spectrometry (PS-MS) is a technique that has recently emerged and has shown excellent analytical sensitivity to a number of drugs in blood. As an alternative to blood, fingerprints have been shown to provide a noninvasive and traceable sampling matrix. Our goal was to validate the use of fingerprint samples to detect cocaine use.

METHODS:

Samples were collected on triangular pieces (168 mm2) of washed Whatman Grade I chromatography paper. Following application of internal standard, spray solvent and a voltage were applied to the paper before mass spectrometry detection. A fingerprint visualization step was incorporated into the analysis procedure by addition of silver nitrate solution and exposing the sample to ultraviolet light.

RESULTS:

Limits of detection for cocaine, benzoylecgonine, and methylecgonine were 1, 2, and 31 ng/mL respectively, with relative standard deviations < 33%. No matrix effects were observed. Analysis of 239 fingerprint samples yielded a 99% true-positive rate and a 2.5% false-positive rate, based on the detection of cocaine, benzoylecgonine, or methylecgonine with use of a single fingerprint.

CONCLUSIONS:

The method offers a qualitative and noninvasive screening test for cocaine use. The analysis method developed is rapid (4 min/sample) and requires no sample preparation.

PMID:
28939761
DOI:
10.1373/clinchem.2017.275578
[Indexed for MEDLINE]
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