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Bio Protoc. 2017 Aug 5;7(15). pii: e2429. doi: 10.21769/BioProtoc.2429.

Mouse Müller Cell Isolation and Culture.

Author information

1
Department of Ophthalmology and Visual Sciences, University of Louisville, Louisville, USA.
2
Department of Ophthalmology, Second Xiangya Hospital of Central South University, Changsha, China.

Abstract

Müller cells are the major supportive and protective glial cells across the retina. Unlike in fish, they have lost the capacity to regenerate the retina in mammals. But, mammalian Müller cells still retain certain retinal stem cell properties with various degree of self-renewal and differentiation potentials, and thereby held a merit in cell-based therapies for treating retinal degeneration diseases. In our laboratory, we use an enzymatic procedure to isolate, purify, and culture mouse Müller cells.

KEYWORDS:

Mouse; Müller glial cells; Primary cell culture; Retinal cell isolation; Stem cells; Worthington Papain Kit

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