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Nat Commun. 2017 Sep 21;8(1):641. doi: 10.1038/s41467-017-00723-0.

IgG1 memory B cells keep the memory of IgE responses.

Author information

1
Singapore Immunology Network (SIgN), 8A Biomedical Grove, Singapore, 138648, Singapore.
2
School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore.
3
Division of Pulmonary, Critical Care and Sleep Medicine, Departments of Medicine and Cell Biology, New York University School of Medicine, 550 First Ave, New York, 10016, USA.
4
Galderma R&D, Les Templiers, 2400 route des Colles, Sophia Antipolis, 06410, Biot, France.
5
Skirball Institute and Department of Pathology, New York University School of Medicine, 540 First Ave, New York, 10016, USA.
6
Singapore Immunology Network (SIgN), 8A Biomedical Grove, Singapore, 138648, Singapore. maria.lafaille@med.nyu.edu.
7
Division of Pulmonary, Critical Care and Sleep Medicine, Departments of Medicine and Cell Biology, New York University School of Medicine, 550 First Ave, New York, 10016, USA. maria.lafaille@med.nyu.edu.

Abstract

The unique differentiation of IgE cells suggests unconventional mechanisms of IgE memory. IgE germinal centre cells are transient, most IgE cells are plasma cells, and high affinity IgE is produced by the switching of IgG1 cells to IgE. Here we investigate the function of subsets of IgG1 memory B cells in IgE production and find that two subsets of IgG1 memory B cells, CD80+CD73+ and CD80-CD73-, contribute distinctively to the repertoires of high affinity pathogenic IgE and low affinity non-pathogenic IgE. Furthermore, repertoire analysis indicates that high affinity IgE and IgG1 plasma cells differentiate from rare CD80+CD73+ high affinity memory clones without undergoing further mutagenesis. By identifying the cellular origin of high affinity IgE and the clonal selection of high affinity memory B cells into the plasma cell fate, our findings provide fundamental insights into the pathogenesis of allergies, and on the mechanisms of antibody production in memory B cell responses.IgE is an important mediator of protective immunity as well as allergic reaction, but how high affinity IgE antibodies are produced in memory responses is not clear. Here the authors show that IgE can be generated via class-switch recombination in IgG1 memory B cells without additional somatic hypermutation.

PMID:
28935935
PMCID:
PMC5608722
DOI:
10.1038/s41467-017-00723-0
[Indexed for MEDLINE]
Free PMC Article

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