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Acta Trop. 2017 Dec;176:380-384. doi: 10.1016/j.actatropica.2017.09.009. Epub 2017 Sep 19.

Anti-Trypanosoma, anti-Leishmania and cytotoxic activities of natural products from Psidium brownianum Mart. ex DC. and Psidium guajava var. Pomifera analysed by LC-MS.

Author information

1
Laboratório de Microbiologia e Biologia Molecular, Universidade Regional do Cariri, Crato, CE, Brazil; Faculdade Vale do Salgado - FVS, Icó, CE, Brazil.
2
Laboratório de Microbiologia e Biologia Molecular, Universidade Regional do Cariri, Crato, CE, Brazil.
3
Center for the Development of Scientific Research, Moisés Bertoni Foundation/Díaz Gill Laboratories, Asunción, Paraguay.
4
Laboratório de Pesquisa de Produtos Naturais, Universidade Regional do Cariri, Crato, CE, Brazil.
5
Centro Universitário Dr. Leão Sampaio - UNILEÃO, Juazeiro do Norte, CE, Brazil.
6
Universidade Federal do Vale do São Francisco - UNIVASF, Petrolina, PE, Brazil.
7
Universidade Federal de Campina Grande - UFCG, Cajazeiras, PB, Brazil.
8
Laboratório de Microbiologia e Biologia Molecular, Universidade Regional do Cariri, Crato, CE, Brazil. Electronic address: hdmcoutinho@gmail.com.

Abstract

Neglected diseases are those that are prevalent in developing countries, even with a rich biodiversity. These diseases still persist because of the lack of scientific studies, government negligence or failures of the public health system. This study aims to identify the composition of extracts and fractions from Psidium brownianum and Psidium guajava through LC-MS, to evaluate its in vitro anti-parasitic and cytotoxic activity against Trypanosoma cruzi, Leishmania brasiliensis and L. infantum epismastigote and promastigote forms, as well as mammalian cells. The results showed the presence of chemical constituents in the two Psidium species as quercetin, myricetin and gallic acid derivatives. The P. brownianum extract and fractions showed low toxicity at all tested concentrations and all samples were effective at the concentration of 1000μg/mL against the parasites, with the extract being the most efficient against the L. infantum promastigote form. The ethanolic extract, and the flavonoid and tannic fractions, from P. guajava showed low toxicity for the fibroblasts. All samples showed effectiveness at the highest concentration tested and the extract was more effective against the promastigote forms tested. The results showed that the species Psidium brownianum and Psidium guajava demonstrated an anti-parasitic activity against the T. cruzi, L. brasiliensis and L. infantum parasite cell lines indicating these species as an alternative therapy given their efficacy in the in vitro assays performed, opening the possibility for new biological studies to further this knowledge through in vivo assays.

KEYWORDS:

Antiparasitic effect; Cytotoxicity; Psidium brownianum; Psidium guajava

[Indexed for MEDLINE]

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