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Clin Epigenetics. 2017 Sep 16;9:101. doi: 10.1186/s13148-017-0404-9. eCollection 2017.

Identification and characterization of interferon signaling-related microRNAs in occult hepatitis B virus infection.

Author information

1
Department of Epidemiology and Statistics, School of Public Health, Jiangsu Key Laboratory and Translational Medicine for Geriatric Disease, Medical College of Soochow University, 199 Renai Road, Suzhou, Jiangsu 215123 China.
2
Guizhou Center for Disease Control and Prevention, Guizhou, China.
3
Zhangjiagang First People Hospital, Suzhou, China.
4
China Huai'an Fourth Hospital, Huai'an, China.
#
Contributed equally

Abstract

BACKGROUND:

Occult hepatitis B virus infection (OBI) is an important risk factor of liver cirrhosis and hepatocellular carcinoma. Type 1 interferon (IFN) signaling-related miRNAs were significantly associated with hepatitis B virus (HBV) infection. However, the characteristics of serum IFN signaling-related miRNAs in OBI remain unclear. Therefore, this study aimed to analyze the expression levels of serum IFN signaling-related miRNAs in OBI and to evaluate their potential values for OBI diagnosis.

METHODS:

Twenty serum samples for training test (10 healthy controls and 10 OBI patients) and 438 validation serum samples from healthy controls, asymptomatic HBsAg carriers (ASC), and chronic hepatitis B (CHB) and OBI patients were collected. Expression levels of 32 IFN signaling-related miRNAs were analyzed in training and validation sets of samples using RT-qPCR.

RESULTS:

Among 32 IFN signaling-related miRNAs, decreased miR-122 levels and increased miR-130a levels were detected in training OBI samples. Furthermore, the results from validation test showed that the mean serum miR-122 and miR-130a level was 2.28 ± 0.96 and 3.11 ± 0.93 in OBI subjects, respectively. Compared to the healthy controls, ASC and CHB patients, miR-122 levels were significantly downregulated, while miR-130a levels were significantly upregulated in OBI patients. ROC analysis indicated that miR-122 + miR-130a could differentiate OBI from healthy controls, ASC, and CHB (≥ 0.87 of AUC).

CONCLUSIONS:

Our study suggested that decreased serum miR-122 level and increased miR-130a level were significantly associated with OBI. Moreover, a combination of miR-122 and miR-130a could be served as a potential marker for OBI diagnosis.

PMID:
28932321
PMCID:
PMC5603019
DOI:
10.1186/s13148-017-0404-9
[Indexed for MEDLINE]
Free PMC Article

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