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Front Microbiol. 2017 Sep 6;8:1679. doi: 10.3389/fmicb.2017.01679. eCollection 2017.

Comparative Genomics of Burkholderia singularis sp. nov., a Low G+C Content, Free-Living Bacterium That Defies Taxonomic Dissection of the Genus Burkholderia.

Author information

1
Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent UniversityGhent, Belgium.
2
Global and Tropical Health Division, Menzies School of Health Research, DarwinNT, Australia.
3
Centre for Animal Health Innovation, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Sippy DownsQLD, Australia.
4
Centre for Understanding and Preventing Infection in Children, Department of Pediatrics, University of British Columbia, VancouverBC, Canada.
5
College of Public Health, Medical and Veterinary Sciences, Australian Institute of Tropical Health and Medicine, James Cook University, TownsvilleQLD, Australia.
6
Tasmanian Institute of Agriculture, University of Tasmania, HobartTAS, Australia.

Abstract

Four Burkholderia pseudomallei-like isolates of human clinical origin were examined by a polyphasic taxonomic approach that included comparative whole genome analyses. The results demonstrated that these isolates represent a rare and unusual, novel Burkholderia species for which we propose the name B. singularis. The type strain is LMG 28154T (=CCUG 65685T). Its genome sequence has an average mol% G+C content of 64.34%, which is considerably lower than that of other Burkholderia species. The reduced G+C content of strain LMG 28154T was characterized by a genome wide AT bias that was not due to reduced GC-biased gene conversion or reductive genome evolution, but might have been caused by an altered DNA base excision repair pathway. B. singularis can be differentiated from other Burkholderia species by multilocus sequence analysis, MALDI-TOF mass spectrometry and a distinctive biochemical profile that includes the absence of nitrate reduction, a mucoid appearance on Columbia sheep blood agar, and a slowly positive oxidase reaction. Comparisons with publicly available whole genome sequences demonstrated that strain TSV85, an Australian water isolate, also represents the same species and therefore, to date, B. singularis has been recovered from human or environmental samples on three continents.

KEYWORDS:

Burkholderia cepacia complex; Burkholderia pseudomallei complex; Burkholderia singularis; comparative genomics; cystic fibrosis microbiology; whole genome sequence

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