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Int J Nanomedicine. 2017 Sep 11;12:6721-6733. doi: 10.2147/IJN.S141888. eCollection 2017.

Silk fibroin/cartilage extracellular matrix scaffolds with sequential delivery of TGF-β3 for chondrogenic differentiation of adipose-derived stem cells.

Author information

1
Department of Spine Surgery, Tianjin Hospital, Tianjin, People's Republic of China.
2
School and Hospital of Stomatology, Tianjin Medical University, Tianjin, People's Republic of China.
3
The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, People's Republic of China.
#
Contributed equally

Abstract

A 3-D scaffold that simulates the microenvironment in vivo for regenerating cartilage is ideal. In this study, we combined silk fibroin and decellularized cartilage extracellular matrix by temperature gradient-guided thermal-induced phase separation to produce composite scaffolds (S/D). Resulting scaffolds had remarkable mechanical properties and biomimeticstructure, for a suitable substrate for attachment and proliferation of adipose-derived stem cells (ADSCs). Moreover, transforming growth factor β3 (TGF-β3) loaded on scaffolds showed a controlled release profile and enhanced the chondrogenic differentiation of ADSCs during the 28-day culture. The S/D scaffold itself can provide a sustained release system without the introduction of other controlled release media, which has potential for commercial and clinical applications. The results of toluidine blue, Safranin O, and immunohistochemical staining and analysis of collagen II expression showed maintenance of a chondrogenic phenotype in all scaffolds after 28-day culture. The most obvious phenomenon was with the addition of TGF-β3. S/D composite scaffolds with sequential delivery of TGF-β3 may mimic the regenerative microenvironment to enhance the chondrogenic differentiation of ADSCs in vitro.

KEYWORDS:

adipose-derived stem cells; cartilage tissue engineering; composite scaffold; decellularized cartilage extracellular matrix; silk fibroin; transforming growth factor β3 (TGF-β3)

PMID:
28932116
PMCID:
PMC5600265
DOI:
10.2147/IJN.S141888
[Indexed for MEDLINE]
Free PMC Article

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