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Sci Rep. 2017 Sep 20;7(1):11977. doi: 10.1038/s41598-017-12358-8.

Exercise training enhances in vivo clearance of endotoxin and attenuates inflammatory responses by potentiating Kupffer cell phagocytosis.

Author information

1
Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, 305-8575, Japan.
2
The Center of Sports Medicine and Health Sciences, Tsukuba University Hospital, Ibaraki, 305-8576, Japan.
3
Japan Society for the Promotion of Science, Tokyo, 102-0083, Japan.
4
Division of Molecular and Cellular Physiology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, 305-8575, Japan.
5
Medical Sciences, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, 305-8575, Japan.
6
Division of Cardiology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, 305-8575, Japan.
7
Division of Gastroenterology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, 305-8575, Japan.
8
Division of Plastic Surgery, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, 305-8575, Japan.
9
Plastic Surgery, Shonai Amarume Hospital, Yamagata, 999-7782, Japan.
10
Division of Oral and Maxillofacial Surgery, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, 305-8575, Japan.
11
The Center of Sports Medicine and Health Sciences, Tsukuba University Hospital, Ibaraki, 305-8576, Japan. shodaj@md.tsukuba.ac.jp.
12
Medical Sciences, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, 305-8575, Japan. shodaj@md.tsukuba.ac.jp.

Abstract

The failure of Kupffer cells (KCs) to remove endotoxin is an important factor in the pathogenesis of non-alcoholic fatty liver disease (NAFLD). In this study, the effects of exercise training on KC function were studied in terms of in vivo endotoxin clearance and inflammatory responses. Mice were allocated into rest and exercise groups. KC bead phagocytic capacity and plasma steroid hormone levels were determined following exercise training. Endotoxin and inflammatory cytokine levels in plasma were determined over time following endotoxin injection. KC bead phagocytic capacity was potentiated and clearance of exogenously-injected endotoxin was increased in the exercise group. Inflammatory cytokine (TNF-α and IL-6) levels were lower in the exercise group. We found that only DHEA was increased in the plasma of the exercise group. In an in vitro experiment, the addition of DHEA to RAW264.7 cells increased bead phagocytic capacity and attenuated endotoxin-induced inflammatory responses. These results suggest that exercise training modulates in vivo endotoxin clearance and inflammatory responses in association with increased DHEA production. These exercise-induced changes in KC capacity may contribute to a slowing of disease progression in NAFLD patients.

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