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Molecules. 2017 Sep 20;22(9). pii: E1578. doi: 10.3390/molecules22091578.

Cytotoxic and Apoptotic Activities of Prunus spinosa Trigno Ecotype Extract on Human Cancer Cells.

Author information

1
National Center for Drug Research and Evaluation, Italian National Institute of Health, Rome 00161, Italy. stefania.meschini@iss.it.
2
National Center for Drug Research and Evaluation, Italian National Institute of Health, Rome 00161, Italy. evelin.pellegrini@guest.iss.it.
3
National Center for Drug Research and Evaluation, Italian National Institute of Health, Rome 00161, Italy. maria.condello@iss.it.
4
Italian Society of Biointegrated Medicine, Bagnoli del Trigno, Isernia 86091, Italy. giovanniocchionero@hotmail.com.
5
Department of Agriculture, Environment and Food Science, University of Molise, Campobasso 86100, Italy. delfine@unimol.it.
6
Department of Movement, Human and Health Sciences, University of Rome Foro Italico, Rome 00135, Italy. giancarlo.condello@gmail.com.
7
Italian Society of Biointegrated Medicine, Bagnoli del Trigno, Isernia 86091, Italy. mastrodonato.f@gmail.com.

Abstract

The aim of this work was to demonstrate that a natural compound, not-toxic to normal cells, has cytotoxic and sensitizing effects on carcinoma cells, with the final goal of combining it with chemotherapeutic drugs to reduce the overall dose. Prunus spinosa Trigno ecotype (PsT) drupe extract with a nutraceutical activator complex (NAC) made of amino acids, vitamins and mineral salt blends, has shown in vitro anticancer activity. The cytotoxic effect of (PsT + NAC)® has been evaluated on human cancer cells, with an initial screening with colorectal, uterine cervical, and bronchoalveolar cells, and a subsequent focus on colon carcinoma cells HCT116 and SW480. The viability reduction of HCT116 and SW480 after treatment with (PsT 10 mg/mL + NAC)® was about 40% (p < 0.05), compared to control cells. The cell's survival reduction was ineffective when the drug vehicle (NAC) was replaced with a phosphate buffer saline (PBS) or physiological solution (PS). The flow cytometry evaluation of cancer cells' mitochondrial membrane potential showed an increase of 20% depolarized mitochondria. Cell cycle analysis showed a sub G1 (Gap 1 phase) peak appearance (HCT116: 35.1%; SW480: 11.6%), indicating apoptotic cell death induction that was confirmed by Annexin V assay (HCT116: 86%; SW480: 96%). Normal cells were not altered by (PsT + NAC)® treatments.

KEYWORDS:

Prunus spinosa; Trigno ecotype; anticancer activity; apoptosis; mitochondrial dysfunction

PMID:
28930188
DOI:
10.3390/molecules22091578
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