Format

Send to

Choose Destination
Neurosci Lett. 2017 Nov 1;660:140-146. doi: 10.1016/j.neulet.2017.09.036. Epub 2017 Sep 18.

The histone deacetylase inhibitor sodium butyrate protects against noise-induced hearing loss in Guinea pigs.

Author information

1
Department of Otolaryngology - Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China; Department of Otolaryngology - Head and Neck Surgery, Fuxing Hospital, Capital Medical University, Beijing, 100038, China.
2
Department of Otolaryngology - Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China.
3
Department of Otolaryngology - Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China. Electronic address: guopengent@163.com.
4
Department of Otolaryngology - Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China. Electronic address: gongss1962@163.com.

Abstract

Noise-induced hearing loss (NIHL) severely impacts the quality of life of affected individuals. Oxidative stress resulting from noise exposure is a significant cause of NIHL. Although histone deacetylase (HDAC) inhibitors were shown to protect against NIHL, the underlying mechanism remains unclear, and it is not known how they act on noise-induced oxidative stress. In the current study, we investigated the expression levels of acetyl-histone H3 (Lys9) (H3-AcK9), histone deacetylase 1 (HDAC1), and 3-nitrotyrosine (3-NT), an oxidative stress marker, in a guinea pig model of NIHL using immunohistology and Western blotting. We then assessed the effects of systemic administration of the HDAC inhibitor, sodium butyrate (SB), on noise-induced permanent threshold shifts (PTS), hair cell (HC) loss, and changes in the above mentioned markers. The results showed that SB attenuated noise-induced PTS and outer hair cell loss. SB treatment promoted H3-AcK9 expression and repressed HDAC1 expression in the nuclei of HCs and Hensen's cells after noise exposure. Furthermore, SB attenuated the noise-induced increase of 3-NT expression in HCs and Hensen's cells. These findings suggest that SB protects against NIHL by reversing the noise-induced histone acetylation imbalance and inhibiting oxidative stress in cochlear HCs and Hensen's cells. SB treatment may represent a potential strategy to prevent and treat NIHL.

KEYWORDS:

Epigenetics; Histone; Histone deacetylase inhibitor; Noise-induced hearing loss; Oxidative stress; Sodium butyrate

PMID:
28928030
DOI:
10.1016/j.neulet.2017.09.036
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center