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J AOAC Int. 2018 May 1;101(3):810-816. doi: 10.5740/jaoacint.17-0036. Epub 2017 Sep 19.

Quantitative Detection of Pork Contamination in Cooked Meat Products by ELISA.

Author information

1
Molecular Epidemiology, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155, USA.
2
IEH Laboratories and Consulting Group, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155, USA.
3
Molecular Epidemiology, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155, USA; IEH Laboratories and Consulting Group, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155, USA.

Abstract

Recent news of many cases of adulteration of meats with pork has bolstered the need for a way to detect and quantify the unwanted contamination of pork in other meats. To address this need, Microbiologique, Inc. has produced a sandwich ELISA assay that can rapidly quantify the presence of pork in cooked horse, beef, chicken, goat, and lamb meats. We carried out a validation study and showed that this assay has an analytical sensitivity of 0.00014 and 0.00040% (w/v) for cooked and autoclaved pork, respectively, and an analytical range of quantitation of 0.05-3.2% (w/v) in the absence of other meats. The assay can measure pork contamination down to 0.1% (w/w) in the presence of cooked horse, beef, chicken, goat, and lamb meats. The assay is quick and can be completed in 1 h and 10 min.

PMID:
28927492
DOI:
10.5740/jaoacint.17-0036

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