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Stem Cell Res. 2017 Oct;24:118-127. doi: 10.1016/j.scr.2017.09.002. Epub 2017 Sep 12.

Laminin α5 substrates promote survival, network formation and functional development of human pluripotent stem cell-derived neurons in vitro.

Author information

1
NeuroGroup, BioMediTech, University of Tampere, Lääkärinkatu 1, 33520 Tampere, Finland. Electronic address: anu.hyysalo@staff.uta.fi.
2
NeuroGroup, BioMediTech, University of Tampere, Lääkärinkatu 1, 33520 Tampere, Finland.
3
Computational Biology, BioMediTech, University of Tampere, Lääkärinkatu 1, 33520 Tampere, Finland.

Abstract

Laminins are one of the major protein groups in the extracellular matrix (ECM) and specific laminin isoforms are crucial for neuronal functions in the central nervous system in vivo. In the present study, we compared recombinant human laminin isoforms (LN211, LN332, LN411, LN511, and LN521) and laminin isoform fragment (LN511-E8) in in vitro cultures of human pluripotent stem cell (hPSC)-derived neurons. We showed that laminin substrates containing the α5-chain are important for neuronal attachment, viability and network formation, as detected by phase contrast imaging, viability staining, and immunocytochemistry. Gene expression analysis showed that the molecular mechanisms involved in the preference of hPSC-derived neurons for specific laminin isoforms could be related to ECM remodeling and cell adhesion. Importantly, the microelectrode array analysis revealed the widest distribution of electrophysiologically active neurons on laminin α5 substrates, indicating most efficient development of neuronal network functionality. This study shows that specific laminin α5 substrates provide a controlled in vitro culture environment for hPSC-derived neurons. These substrates can be utilized not only to enhance the production of functional hPSC-derived neurons for in vitro applications like disease modeling, toxicological studies, and drug discovery, but also for the production of clinical grade hPSC-derived cells for regenerative medicine applications.

KEYWORDS:

Alpha5 laminin substrate; Human pluripotent stem cell-derived neuron; In vitro culturing; Microelectrode array; Neuronal networks

PMID:
28926760
DOI:
10.1016/j.scr.2017.09.002
[Indexed for MEDLINE]
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