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Oncogene. 2018 Jan 18;37(3):302-312. doi: 10.1038/onc.2017.341. Epub 2017 Sep 18.

A slow-cycling subpopulation of melanoma cells with highly invasive properties.

Author information

1
Melanoma Research Center, The Wistar Institute, Philadelphia, PA, USA.
2
Division of Immunology, Allergy and Infectious Diseases, Medical University of Vienna, Vienna, Austria.
3
Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, USA.
4
CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.
5
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, USA.
6
Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.

Abstract

Melanoma is a heterogeneous tumor with different subpopulations showing different proliferation rates. Slow-cycling cells were previously identified in melanoma, but not fully biologically characterized. Using the label-retention method, we identified a subpopulation of slow-cycling cells, defined as label-retaining cells (LRC), with strong invasive properties. We demonstrate through live imaging that LRC are leaving the primary tumor mass at a very early stage and disseminate to peripheral organs. Through global proteome analyses, we identified the secreted protein SerpinE2/protease nexin-1 as causative for the highly invasive potential of LRC in melanomas.

PMID:
28925403
PMCID:
PMC5799768
DOI:
10.1038/onc.2017.341
[Indexed for MEDLINE]
Free PMC Article

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