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Stem Cell Res. 2017 Aug;23:127-131. doi: 10.1016/j.scr.2017.07.007. Epub 2017 Jul 11.

Generation of a KLF15 homozygous knockout human embryonic stem cell line using paired CRISPR/Cas9n, and human cardiomyocytes derivation.

Author information

1
Institute of Pharmacology and Toxicology, University Medical Center Goettingen, Germany; DZHK (German Center for Cardiovascular Disease), Partner Site Goettingen, Germany.
2
Clinic for Cardiology and Pneumology, University Medical Center Goettingen, Germany; DZHK (German Center for Cardiovascular Disease), Partner Site Goettingen, Germany.
3
Institute of Pharmacology and Toxicology, University Medical Center Goettingen, Germany; DZHK (German Center for Cardiovascular Disease), Partner Site Goettingen, Germany. Electronic address: laura.zelarayan@med.uni-goettingen.de.

Abstract

Krueppel-like factor 15 (KLF15) is abundantly expressed in liver, kidney, and muscle, including myocardium. In the adult heart KLF15 is important to maintain homeostasis and to repress hypertrophic remodeling. We generated a homozygous hESC KLF15 knockout (KO) line using paired CRISPR/Cas9n. KLF15-KO cells maintained full pluripotency and differentiation potential as well as genomic integrity. We demonstrated that KLF15-KO cells can be differentiated into morphologically normal cardiomyocytes turning them into a valuable tool for studying human KLF15-mediated mechanisms resulting in human cardiac dysfunction.

PMID:
28925362
DOI:
10.1016/j.scr.2017.07.007
[Indexed for MEDLINE]
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