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Appl Microbiol Biotechnol. 2017 Nov;101(21):7771-7779. doi: 10.1007/s00253-017-8484-1. Epub 2017 Sep 18.

Keratinolytic protease: a green biocatalyst for leather industry.

Fang Z1,2, Yong YC1, Zhang J3,4, Du G2,5, Chen J2,6.

Author information

1
Biofuels Institute, School of the Environment, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu Province, 212013, China.
2
School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, China.
3
School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, China. zhangj@jiangnan.edu.cn.
4
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China. zhangj@jiangnan.edu.cn.
5
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China.
6
National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China.

Abstract

Depilation/unhairing is the crucial but heavy pollution process in leather industry. Traditional inorganic sulfide treatment was the most widely used depilation technique in the past decades, which was usually detrimental to leather quality and resulted in serious environmental pollution. Using biocatalysts to substitute inorganic sulfide showed great advantages in environment protection and unhairing efficiency. Keratinolytic protease is one of the excellent biocatalysts to hydrolyze disulfide bond-rich proteins of hair and has little damage to leather. Biological treatment with keratinolytic proteases could largely reduce the quantity and toxicity of wastewater effluent from the leather industry. But low thermostability and substrate specificity or specific activity of these enzymes limited their practical application. Therefore, recent progresses on protein engineering strategies (site-directed mutagenesis, protein fusion, N/C-terminus truncation, and domain swapping) used to enhance the keratinolytic enzyme performance were presented.

KEYWORDS:

Enzymatic catalyst; Industrial biotechnology; Keratinolytic proteases; Lime sulfide free; Protein engineering; Unhairing process

PMID:
28924866
DOI:
10.1007/s00253-017-8484-1
[Indexed for MEDLINE]

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